动物造模,帕金森病 z-bio 2022-09-05 247

　　Objective To observe the effect of substantia nigra overexpressing lysosome-associated membrane protein 2A up-regulating molecular chaperone-mediated autophagy on the motor behavior of experimental monkeys in a rhesus monkey model of Parkinson's disease.

　　METHODS: Seven rhesus monkeys were injected with Lewy bodies (LBs) extracts from postmortem brain tissue (voluntary donation) of PD patients in the right striatum region of seven rhesus monkeys. The PD rhesus monkey model was constructed by injecting extracts of brain tissue (voluntary donation) from non-PD patients who died. The model animals were randomly divided into two groups. The LAMP2A overexpression group (4 animals) was injected bilaterally with the adeno-associated virus vector overexpressing LAMP2A in the substantia nigra; the control group (3 animals) was injected with the control virus vector bilaterally in the substantia nigra. The motor ability of the PD rhesus monkey model and the effect of LAMP2A on the motor function of the PD model were evaluated by the ladder test and the plate feeding test. The levels of total α-synuclein (α-syn) and phosphorylated α-syn in cerebrospinal fluid and serum of experimental monkeys were determined by ELISA.

　　Results The fine motor level of the left upper limbs of the rhesus monkeys in the control group decreased compared with that of the right side in the ladder experiment and the plate feeding experiment; the fine motor levels of the left and right upper limbs of the rhesus monkeys in the LAMP2A overexpression group did not show significant difference. The levels of total α-syn and phosphorylated α-syn in cerebrospinal fluid and serum in the control group were significantly increased at 12 months after operation by ELISA; Months later were lower than the control group in the same period.

　　Conclusions Overexpression of LAMP2A may improve fine motor ability in a PD rhesus monkey model by increasing CMA clearance of α-syn.