动物造模,胰腺癌原位模型 z-bio 2022-09-05 408

　　Objective To explore the advantages and disadvantages of using tumor cell orthotopic injection and tumor tissue block transplantation to establish a mouse pancreatic cancer orthotopic model, and to provide technical reference for the establishment of a mouse pancreatic cancer orthotopic model for the purpose of precise ablation therapy.

　　Methods Twenty healthy male C57BL/6 mice aged 6-8 weeks were divided into 4 groups with 5 mice in each group: group A was directly injected with mouse pancreatic cancer Panc02 cell suspension; group B was treated with Panc02 cell suspension injection method of mouse pancreatic cancer; group C using mouse pancreatic cancer tissue block transplantation method; group D using mouse tumor tissue block hydrogel bonding method. The tumor formation rate, tumor size, degree of adhesion of abdominal organs and tumor metastasis of pancreatic cancer in mice in each group were observed after operation. HE staining and immunohistochemistry were used to detect the morphological changes of tumor tissue and the activity of Ki67 and α smooth muscle. Differential expression of protein (α-smooth muscle actin, α-SMA).

　　Results The tumor formation rates of pancreatic cancer in mice in groups A, B, C, and D were 60%, 80%, 100%, and 100%, respectively. After 16 days of modeling, the tumor volume of mice in group A was (47.80±42.99) mm3, with poor homogeneity; the tumor volumes of mice in groups B, C and D were (68.43±16.77) mm3, (105.86±17.25) mm3 and (128.98±13.41) mm3, which was significantly larger than that of group A (P<0.01), and="" the="" homogeneity="" was="" better.="" severe="" intraperitoneal="" adhesion="" rate="" of="" mice="" in="" group="" a="" that="" b="" there="" no="" c="" d.="" tumor="" metastasis="" found="" were="" significant="" histological="" differences="" tumors="" four="" groups="" difference="" relative="" expression="" levels="" ki67="" -sma="" proteins="" tissues="" p="">0.05).

　　Conclusion The mouse pancreatic cancer orthotopic model established by tumor tissue block transplantation and hydrogel bonding has high tumor formation rate, less metastasis, less intraperitoneal adhesion, and does not change the biological characteristics of tumor cells. It is easy to operate and popularize. The modeling effect is better.