动物造模,心肌损伤模型 z-bio 2022-09-05 331

　　Objective To explore the method of using isoproterenol to induce myocardial injury in rhesus monkeys.

　　Methods Eleven rhesus monkeys were selected and divided into high-dose group, low-dose group and control group. The doses of ISO per injection in the model group were 4.6 mg/kg and 3.2 mg/kg, respectively, twice a day; the control group was injected with 1 mL of normal saline, once a day. Color Doppler ultrasonography was performed on the 1st, 3rd, 5th, 7th days and 2nd, 3rd, 4th, and 5th weeks after injection: left ventricular end-diastolic septal thickness (IVSTd), left ventricular diameter (LVEDD) , Left Ventricular Posterior Wall Thickness (LVPWTd), End-systolic Septal Thickness (IVSTs), Left Intraventricular Diameter (LVESD), Left Ventricular Posterior Wall Thickness (LVPWTs), Left Ventricular Short-axis Shortening Rate (FS), Ejection Fraction (EF), left ventricular mass (LVM); electrocardiograph to check the changes of ECG; at the same time, alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine creatase (CK), creatine creatase isoenzyme (CK-MB) ), lactate dehydrogenase (LDH), platelet/lymphocyte ratio (PLR), neutrophil/lymphocyte ratio (NLR) and other blood physiological and biochemical indicators.

　　Results In the high-dose group, AST, ALT, LDH, CK, CK-MB, PLR, and NLR were significantly increased within 1 week of administration; IVSTd, IVSTs, LVPWTd, LVPWTs, LVEDD, and LVESD were increased, while EF and FS were decreased. Compared with the high-dose group, the low-dose group did not change significantly. The ECG results showed that the T wave and ST segment changed to varying degrees from the 5th week after administration.

　　Conclusion Subcutaneous injection of ISO can induce myocardial injury in rhesus monkeys, and the comprehensive indexes of echocardiography, electrocardiogram and hematology can be used as the basis for the evaluation of non-invasive heart injury model in rhesus monkeys.