动物造模,基因敲除 z-bio 2022-09-05 307

　　Objective To establish a liver-specific LCMT1 knockout mouse model.

　　Methods Liver-specific LCMT1-KO mice were constructed using CRISPR/Cas9 technology. The differences between wild and knockout mice were identified and compared by RT-PCR, real-time quantitative PCR, Western Blot and HE staining; the general condition, reproductive ability and survival rate of offspring of the two groups of mice were observed and analyzed.

　　Results The genotype of the offspring was successfully identified; the liver LCMT1 mRNA and protein levels of the LCMT1-KO mice were significantly lower than those of the control mice; the diet, water, body weight, reproductive ability, survival rate of offspring, liver appearance and There was no significant difference in HE staining; LCMT1 expression in the heart, brain and kidney tissues of LCMT1-KO mice did not change significantly compared with the control group; sgRNA in LCMT1-KO mice was not off-target.

　　Conclusion The liver-specific LCMT1 gene knockout mice were successfully constructed, which provides an experimental means for studying the regulatory role of LCMT1 gene in diseases.