动物造模,糖尿病肾病模型 z-bio 2022-09-06 410

　　Objective To compare and study the stability of C57BL/6J mice model of diabetic nephropathy induced by different doses of streptozotocin combined with high-sugar and high-fat diet.

　　Methods Forty-eight male C57BL/6J mice were randomly divided into: control group (injected with equal volume of citrate buffer, 12 mice), single high-dose (150 mg/kg STZ, single intraperitoneal injection combined with high-fat and high-glucose, 18 only), multiple small doses (50 mg/kg STZ, continuous injection for 4 days combined with high-fat and high-sugar, 18 rats). Changes in fasting blood glucose (FBG), water intake, blood lipids, 24-hour urine protein, renal function and renal pathology were detected by the two modeling methods.

　　Results After the injection of STZ, the weight of the mice in the single high-dose group increased slowly, while the weight of the mice in the multiple low-dose group showed a negative increase; the FBG and water intake of the single high-dose mice reached the peak 4 weeks after the injection of STZ, and the 24-hour urine protein was at The peak value was reached after 6 weeks of STZ injection, and then gradually decreased; FBG, 24h urine protein and water intake in the multiple low-dose group increased steadily and maintained at a high level; at the end of the experiment, the FBG, 24h urine protein, 24h urine protein and water intake of the mice in the multiple low-dose group were Serum triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDL), serum creatinine (Scr), serum urea nitrogen (BUN) , kidney index (kidney index, KI) and glomerular reducing sugar levels were significantly higher than the single high-dose group (P<0.05), and the renal pathological changes were more obvious.

　　Conclusion Multiple low-dose injections of STZ combined with a high-sugar and high-fat diet to establish diabetic nephropathy in mice have a high model rate, low mortality, and good stability, and have similar pathogenesis and pathological characteristics of human diabetic nephropathy. method.