动物实验,消化器官发育缺陷 z-bo 2020-08-20 584

　　Objective: To carry out forward genetic screening of mutants with abnormal development of zebrafish liver, intestine and gallbladder.

　　Method: ENU mutagenesis of wild-type zebrafish, classic F2 generation screening, whole embryo in situ hybridization using lfabp as a probe, and BES detection of the liver, intestine and gallbladder of early zebrafish embryos-running H2O2-Ac fluorescent dye.

　　Result: 23 zebrafish gastrointestinal defect mutants of 14 F2 families were screened from 128 mutant genomes, and they were divided into 6 categories according to their phenotypes.

　　Conclusion: The liver, intestine and gallbladder of zebrafish have similar and different regulatory mechanisms.