动物造模,高糖诱导海马神经元模型 z-bio 2022-09-06 342

　　Objective To compare the advantages and disadvantages of two high-glucose-induced hippocampal neuron models, and to explore the application effects of the two cell models in different situations.

　　Methods Primary hippocampal neurons and HT-22 hippocampal neuron cell line were cultured in vitro and divided into control group and high glucose group. Neuron purity identification, cell viability detection, the effect of transfection reagents on cell viability and transfection efficiency, flow cytometry to observe cell apoptosis, Western blot to detect apoptotic protein expression.

　　Results The purity of primary hippocampal neurons was more than 85%; two high-glucose-induced neuron cell models were successfully replicated; the activity of primary hippocampal neurons was stabilized at 80% and the activity of HT-22 cells reached 95% after high glucose exposure for 48 h. The above; different kinds of liposome transfection reagents can damage the two cell models, LipoRNAiMax is less toxic, of which HT-22 cells are less damaged, and the transfection efficiency of HT-22 cells is higher; the two models have significant cell apoptosis ( P < 0. 05), the apoptosis rate of primary neurons was higher, the expression of apoptosis protein Bcl-2 was decreased, and the expression of Bax was increased in primary neurons (P < 0. 05). Bax expression in HT-22 cells No difference.

　　Conclusion The two high-glucose-induced hippocampal neuron cell models have different advantages and disadvantages, and the selection of cell models should be considered according to the actual situation.