动物造模,间质性膀胱炎模型 z-bio 2022-09-06 270

　　Objective To explore the ideal dose of intraperitoneal injection of cyclophosphamide to establish a rat model of interstitial cystitis.

　　Methods Rats were randomly divided into seven groups, and intraperitoneal injection of normal saline as control or 50 mg/kg, 100 mg/kg, 150 mg/kg, 200 mg/kg, 250 mg/kg and 300 mg/kg cyclophosphamide was used to induce the establishment of interstitium. Cystitis model. The inflammatory effects of different doses of cyclophosphamide on bladder tissue were evaluated from histological and molecular levels by HE staining and Real-time PCR. The bladder function and sensation were assessed by urodynamics, behavioral scale and Von-Frey test. Comprehensive evaluation of the modeling situation of rats in each group.

　　Results There were no significant differences in bladder inflammation indexes and spontaneous hyperalgesia scores in the 50 mg/kg group compared with the control group. Compared with the control group, the 100 mg / kg group had significantly higher bladder inflammation indexes (P < 0.05), but there was no significant difference in spontaneous hyperalgesia compared with the control group; 150 mg / kg and 200 mg / kg rats bladder inflammation indexes Compared with the control group, it was significantly higher (P < 0. 05), and spontaneous hyperalgesia occurred (P < 0. 05). The 200 mg / kg group observed that the rats died within 48 hours after CYP injection; 250 mg / kg Compared with the control group, the bladder inflammation index and spontaneous pain threshold were significantly higher in the 300 mg/kg and 300 mg/kg groups (P < 0. 05), but the bladder contractility was impaired (P < 0. 05). Death within 48 hours after cyclophosphamide injection.

　　Conclusion Intraperitoneal injection of cyclophosphamide (150 mg/kg) is an ideal method to establish a rat model of interstitial cystitis, which can simultaneously simulate the two major characteristics of interstitial cystitis: bladder inflammation and spontaneous pain, while taking into account the two characteristics of interstitial cystitis. Modeling efficiency and rat survival.