动物造模 z-bio 2022-09-06 343

　　Objective To construct orthotopic and subcutaneous human tumor xenograft models of hepatocellular carcinoma, and to compare and analyze the growth and pathological characteristics of the two models.

　　Methods Fresh tumor tissues from patients with liver cancer were collected, and the liver and subcutaneous tissues were inoculated. The growth characteristics, pathological characteristics, tumor formation rate and tumor formation time of the two were compared and analyzed.

　　Results In this study, the orthotopic and subcutaneous PDX models of liver cancer were successfully constructed, both of which were consistent with the pathological characteristics of the patient's primary tumor. ), the average tumor formation time was 30 d and 68 d.

　　Conclusions The orthotopic PDX model of liver cancer has a high tumor formation rate, which provides a new modeling method for difficult-to-grow tumors. The operation and observation of subcutaneous tumor formation are relatively simple, and appropriate methods should be used according to the situation in specific scientific research practice. They provide effective animal models for in-depth exploration of the pathogenesis of HCC and drug screening.