动物造模,胆酸诱导急性肺损伤小鼠模型 z-bio 2022-09-06 466

　　Objective To construct a mouse model of bile acid-induced acute lung injury, screen the administration methods, and explore the feasibility of bile acid-induced lung injury.

　　Methods The mice were divided into groups according to the factorial design of administration method × drug (3 × 3). The administration methods included tracheotomy, nasal instillation for 1 day and nasal instillation for 6 days. The drug was bile acid and its dissolution control DMSO and blank control PBS. , so a total of 9 groups of mice. Body weight changes were monitored during administration, X-ray plain film examination of chest was performed after administration, pathological changes of lung tissue were observed by histology, arterial blood was collected to analyze partial pressure of oxygen (PO2), and ELISA was used to detect tumor necrosis in lung tissue. Factor (TNF-α) and interleukin-1β (IL-1β) content.

　　Results X-ray plain films of the tracheotomy-reperfusion bile acid group showed diffuse infiltration of lung tissue, gross lung samples showed obvious hemorrhage, and histological pathology showed a large number of inflammatory cell infiltration and alveolar wall thickening, blood oxygen partial pressure. TNF-α and IL-1β were significantly higher than other groups. The changes of each index in the 1-day nasal instillation cholic acid group and the nasal infusion 6-day cholic acid group were less than those in the tracheotomy-reperfusion cholic acid group.

　　Conclusion Tracheotomy and perfusion of bile acid can successfully establish a mouse model of acute lung injury.