动物造模,兔颈总动脉粥样硬化斑块模型 z-bio 2022-09-06 312

　　Objective To explore a new method for establishing a rabbit common carotid atherosclerosis model by using balloon-injured common carotid artery intima and feeding with high-fat diet through the central artery of rabbit ear, and to evaluate the feasibility.

　　Methods The rabbits were pre-fed with high-fat diet for 4 weeks, then the intima of the right common carotid artery was injured by balloon through the central artery puncture of rabbit ears, and then fed with high-fat diet for 8 weeks. The lesions of common carotid artery in rabbits were observed by magnetic resonance apparatus and histopathology.

　　Results The success rate of constructing an animal model of common carotid atherosclerosis in rabbits in this study was 85%. On magnetic resonance images, the common carotid artery wall in the experimental group was thickened and significantly enhanced, and the signal intensity and signal-to-noise ratio of atherosclerotic plaques in the experimental group were significantly higher than those in the control group. The intima of the common carotid artery in the experimental group showed pathological manifestations of atherosclerotic plaque, while the intima of the common carotid artery in the control rabbit was normal.

　　Conclusion The rabbit common carotid atherosclerotic plaque model was successfully constructed by the method of balloon injury of the intima of the common carotid artery of the rabbits combined with high-fat diet through the puncture of the central artery of the rabbit ear. It has the advantages of strong repeatability, short molding time and high molding rate. Balloon injury to the intima of the common carotid artery adopts the original puncture approach of the central artery of the rabbit ear, which opens up a new idea for the establishment of a rabbit common carotid atherosclerotic plaque model.