动物造模,膝骨性关节炎模型 z-bio 2022-09-07 292

　　Objective To use monoiodoacetic acid to induce a rat model of knee osteoarthritis, to detect and evaluate it at different time points, and to analyze the similarity with the pathogenesis of clinical OA.

　　Methods Twenty-four 2-month-old male SD rats were randomly divided into OA model group (18 rats) and normal control group (6 rats). After 3 days of adaptive feeding, rats in the model group were injected with 2 mg MIA (dissolved in 50μ normal saline) in the right knee joint cavity, while the rats in the control group were injected with the same amount of normal saline in the right knee joint cavity. The rats in the model group and the control group were sacrificed at 4 weeks, and the knee articular cartilage tissue samples of the rats in each group were collected for morphological observation, histological staining and OA cartilage histopathological assessment system (OOCHAS) score.

　　Results The rats in the model group had articular cartilage damage after 1 week, 2 weeks and 4 weeks after modeling, the number of chondrocytes was reduced, the tide line was blurred, the staining of toluidine blue and safranin O became lighter, and the trend was progressive. ; The OOCHAS gross score and histological score of rats in the model group increased significantly with time, 1 week and 2 weeks after modeling were equivalent to the early and middle stages of the disease, and 4 weeks were equivalent to the late stage of the disease.

　　Conclusion The knee OA rat model was successfully induced by intra-articular injection of MIA, and the results at different time points were correlated with the clinical disease process, laying a foundation for later functional and mechanism studies.