动物造模,高原肺水肿模型 z-bio 2022-09-07 443

　　Objective To study the effect of different hypoxic stress methods in constructing high altitude pulmonary edema model.

　　Methods Sixty SD rats were randomly divided into 3 groups: control group (altitude 400m), hypoxic chamber group (simulated hypoxia stress at 6000m altitude for 48h), field hypoxia group (hypoxia stress at 4200m altitude for 28d); Group of 20. By detecting the dry-to-moisture ratio, morphological and pathophysiological characteristics, the expression of key genes aquaporin-1 (AQP-1) and vascular endothelial growth factor (VEGF) in the lung tissue of experimental rats, and The oxidative stress level was compared, and the effects of different hypoxic stress methods in constructing the SD rat model of high altitude pulmonary edema were compared.

　　Results Compared with the control group, the pulmonary arterial pressure and lung tissue water content of the rats in the hypobaric oxygen chamber group and the field hypoxia group were significantly increased (both P<0.01), while the oxygen partial pressure and oxygen saturation were significantly decreased (both P<0.01). <0.01). The morphology of the lung tissue in the control group showed normal structure under light microscope and electron microscope; the alveolar wall and the alveolar septum were obviously widened in the lung tissue of the hypobaric oxygen chamber group and the field hypoxia group under the light microscope, and a large number of red blood cells and inflammatory cells overflowed. And there was obvious edema in the alveolar septa. Compared with the control group, the levels of AQP-1 mRNA and protein in the lung tissue of the two experimental groups were significantly increased (both P<0.01), and the VEGF mRNA and protein levels were significantly lower than those of the control group (both P<0.01). The levels of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were significantly decreased, and the levels of malondialdehyde (MDA) were significantly increased.

　　Conclusion The hypoxic chamber simulated hypoxia stress at an altitude of 6000m for 48 hours, and the hypoxia stress at an altitude of 4200m for 28 days can effectively build the SD rat model of high altitude pulmonary edema. Among them, the use of the hypobaric oxygen chamber to build the SD rat high altitude pulmonary edema model is relatively more Advantage.