动物造模,颅骨缺损动物模型 z-bio 2022-09-07 304

　　Objective To explore the ideal method for constructing the rat skull defect model and improve the success rate of the animal model of bone defect.

　　Methods 40 adult male SD rats were selected and randomly divided into control group and experimental group. In the control group, the traditional skull defect model operation method was used, only a hollow trephine drill was used to drill the full-thickness defect of the skull with force perpendicular to the surface of the skull. The group applied a modified surgical method, using both hollow and solid drill bits, and changing the force direction of the drill bits at different stages of the process of drilling the skull. The surgical drilling time of the two groups of models was compared, and whether there were complications such as periosteum, dura mater, brain tissue damage and hemorrhage during the operation, and the alumina bioceramic material implanted in the two groups of models was observed 8 weeks after the operation. , loosening and osseointegration, and the success rate of modeling was compared between the two groups.

　　Results Compared with the control group, the surgical drilling time in the experimental group was significantly shortened (P<0.01), the intraoperative periosteum, dura mater, brain tissue damage and hemorrhage and other complications were significantly reduced (P<0.05). There was no displacement and loosening of the ceramic material (P<0.05), the material was well integrated with the skull, and the success rate of modeling was significantly improved (P<0.05).

　　Conclusion The improved rat skull defect model construction method has the advantages of short surgical drilling time and fewer complications, which can greatly improve the success rate of modeling and can effectively evaluate the bone repair performance of orthopedic implant materials in vivo.