动物造模 z-bio 2022-09-07 427

　　Objective To establish a mouse model of bone metastasis by intracardiac injection of human lung cancer cells by ultrasound guidance.

　　Methods Under the guidance of ultrasound, human lung cancer A549 and NCl-H1299 cells were injected into the left ventricle of NOD/SCID and BALB/c-nu/nu mice, respectively. Small animal imaging methods were used to observe the establishment of the model and the occurrence of bone metastases. The pathological observation of tumor tissue was performed by HE staining.

　　Results All animals survived intracardiac injection of lung cancer cells under ultrasound guidance. In vivo imaging of small animals showed that there were luminescent areas in the axial and limb bones of mice. The three-dimensional reconstruction of the whole body skeleton of mice by X-ray and small animal Micro-CT showed that obvious bone and periosteal defects and destruction occurred in the tibial plateau and shoulder joint of the mice, and the normal bone structure disappeared, showing osteolytic The imaging findings are consistent with the changes of tumorous bone destruction. Gross anatomy showed that the axial bone and limb bones and joints of the mice were wrapped by tumor tissue, and bone metastases appeared in different degrees.

　　Conclusion Ultrasound-guided intracardiac injection of lung cancer bone metastases model can effectively improve the success rate of modeling, more realistically simulate the biological characteristics of clinical lung cancer patients with bone metastases, and can provide experiments for the study of lung cancer metastasis mechanism, anti-metastatic therapy and individualized therapy.