动物造模,非酒精性脂肪肝病 z-bio 2022-09-07 305

　　Objective To establish an animal model of high-fat diet-induced non-alcoholic fatty liver disease in cynomolgus monkeys, to analyze the effect of high-fat diet feeding time on the formation of NAFLD in animals, and to explore the relationship between high-fat diet feeding time and serum biochemical indicators, liver steatosis and fibrosis formed correlation.

　　Methods Before the experiment, basal blood was collected from 700 healthy cynomolgus monkeys, and then they were fed with high-fat diet. After feeding for 1 year, 2 years, 3 years, 4 years, 5 years, 6 years and 7 years, randomly selected Some experimental monkeys were anesthetized with ketamine hydrochloride and the blood of the animals was collected to detect serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), High-density lipoprotein (HDL), low-density lipoprotein (LDL) indicators, and liver biopsy.

　　Results When fed with high-fat diet for 2 years, the serum levels of ALT, AST, TG, TC, and LDL in cynomolgus monkeys were significantly increased, and the level of HDL was significantly decreased (P<0.01). High-fat diet feeding for 2 years or more was significantly associated with ALT, AST, TG, TC, adipose, and fibrosis at the 0.01 level (two-sided) (r=0.127, 0.121, 0.246, 0.128, 0.306, and 0.220, respectively) , was negatively correlated with HDL (r=0.298, P<0.05), and significantly correlated with LDL at the 0.05 level (bilaterally (r=-0.081). Histopathological observations showed that over time severe steatosis and Balloon degeneration, fatty liver, and then hepatitis and fibrosis.

　　Conclusion High-fat diet can significantly increase blood lipids and liver enzyme activity indexes in cynomolgus monkeys, as well as hepatic lipid accumulation and inflammatory infiltration, indicating that high-fat diet can successfully induce NAFLD cynomolgus monkey model and promote the progression of NAFLD.