动物造模 z-bio 2022-09-07 336

　　Objective By analyzing the relationship between the syndrome of qi deficiency and blood stasis syndrome of coronary heart disease in rats combined with the pathology, etiology, tongue manifestations and the relationship with prostacyclin and thromboxane A2, to explore the formation of the rat model of CHD combined with qi deficiency and blood stasis syndrome. model method.

　　Methods Thirty-two rats were randomly divided into 4 groups: K group: normal diet and drinking water (n=8); L group: ligation of left anterior descending coronary artery (n = 8) after normal feeding for 21 days; Y group: diet control Combined fatigue exercise for 21 days (n = 8); Group F: diet control combined with fatigue exercise for 21 days + ligation of left anterior descending coronary artery (n = 8). After modeling, Photoshop 6.0 was used to analyze the RGB values of the tongue surface, and the "RGB data distribution table" was used to evaluate the tongue color of each model; HE staining was used to detect the height of the filiform papillary epithelium, keratinized layer, lamina propria, and lamina propria microvessels. Quantity; immunohistochemical analysis of PGI2, TXA2 positive expression rate and calculation of T/P ratio.

　　Results (1) The tongue quality of the rats in the K group was classified as "light red tongue"; the R value of the tongue surface of the rats in the L group was significantly lower than that of the K group, and belonged to the "dark red tongue"; the R value of the tongue surface of the rats in the Y group was higher than that of the K group The R, G, and B values of the tongue surface of the rats in the F group were significantly lower than those in the K and L groups, which belonged to the "purple tongue"; (2) the tongue surface of the L group was silky There was no difference in the height of each layer and the number of microvessels in group K; compared with group K, the height of keratinized layer of filiform papilla was significantly lower in group Y; the height of epithelial layer and keratinized layer of filiform papilla in group F were significantly lower than those in group K and L group, the number of microvessels was significantly higher than that of K and L groups; there was no difference in the height of the lamina propria of the filiform papilla in each group; ( 3) The changes of PGI2 in L, Y, and F groups were not obvious compared with K group; L group The TXA2 level and T/P ratio were significantly higher than those in K group; the TXA2 level and T/P ratio in F group were significantly higher than those in K group and L group.

　　Conclusion The RGB numerical characteristics of tongue color in CHD syndrome of qi deficiency and blood stasis are related to the reduction of the height of the filiform papillary epithelial layer and the keratinized layer, and the increase of the number of capillaries in the lamina propria, and the molecular biological mechanism may be related to the imbalance of TXA2/PGI2. The model is more in line with the characteristics of the combination model of CHD qi deficiency and blood stasis syndrome.