动物造模,门脉高压动物模型 z-bio 2022-09-07 341

　　Objective To explore whether simple left portal vein ligation can form stable portal hypertension and whether nitric oxide plays a corresponding role in it.

　　Methods Thirty-six male 8-week-old SD rats of SPF grade were randomly divided into left branch ligation group (respectively ligated with 0. ligation), left lateral lobectomy group, and sham operation group, a total of 6 groups. The portal pressures of all rats were measured before operation, immediately after operation, 15 days after operation, and 30 days after operation. All rats were sacrificed 30 days after operation, and portal blood and left and right hepatic lobes were collected to measure their nitric oxide (NO, nitric oxide) content.

　　Results The portal venous pressure of SD rats in the left branch ligation group was significantly increased immediately after operation, 15 d, and 30 d after operation compared with the control group; 30 d after operation, the portal venous pressure in the left branch 0. There was no significant difference in NO content in left and right lobe of liver tissue and blood in six groups of SD rats.

　　Conclusion Simple left portal branch ligation can form stable portal hypertension, and a needle with a diameter of 0.6 mm can achieve better results; NO plays no role in prehepatic portal hypertension.