动物造模,肝纤维化模型 z-bio 2022-09-07 411

　　Objective To compare the effects of different doses of carbon tetrachloride in inducing hepatic fibrosis in inbred C57BL/6 mice, so as to establish a stable hepatic fibrosis model.

　　Methods The 5-week-old C57BL/6 inbred mice were selected and given high, medium and low doses of carbon tetrachloride by intraperitoneal injection to induce liver fibrosis. After the experiment, blood and liver tissue were collected to detect plasma alanine aminotransferase. (ALT), aspartate aminotransferase (AST) levels and liver pathological changes.

　　Results After the inbred C57BL/6 mice were induced by drugs, the animals in each dose group could survive for up to 8 weeks, and the survival rate in the high dose group was only 20%, which was much lower than that in the middle and low groups. The pathological changes have a dose-time effect relationship. Pathological analysis showed that hepatocyte degeneration/necrosis, mixed cell infiltration around central vein/portal duct area and liver fibrosis were observed in all dose groups.

　　Conclusion 10% carbon tetrachloride administration can induce the inbred C57BL/6 mice to form a stable liver fibrosis mouse model after 8 weeks of administration, which lays a foundation for the follow-up study of liver fibrosis mechanism and drug screening.