动物造模,IBS内脏高敏幼鼠模型 z-bio 2022-09-08 244

　　Objective To explore an efficient method for establishing and evaluating a mouse model of endometriosis and fibrosis.

　　Methods The BALB/c mouse model of endometriosis and fibrosis was established by the modified intraperitoneal injection method; the peritoneal lavage fluid of the mice was collected, and the level of TGF-β1 in the lavage fluid was detected by ELISA kit; the degree of intraperitoneal adhesion of the mice was scored. ; HE staining to verify the success of the endometriosis model; Masson staining to detect the deposition of collagen fibers in ectopic foci to evaluate the degree of collagen deposition; immunohistochemistry to detect fibrosis-related proteins E-cadherin, Collagen I, α-SMA , the expression of smooth muscle myosin heavy chain II (SMMHC-II) to evaluate the degree of ectopic foci of fibrosis.

　　Results The improved intraperitoneal injection method to construct a mouse model of endometriosis and fibrosis had a success rate of 100%. The level of TGF-β1 in the peritoneal lavage fluid of mice increased significantly with the prolongation of modeling time. Masson staining and immunohistochemical staining of fibrosis-related proteins observed fibrotic phenotype in the abdominal cavity of endometriosis mice.

　　Conclusion The improved intraperitoneal injection method has a high success rate in constructing a mouse model of endometriosis and fibrosis. The peritoneal lavage fluid TGF-β1 level, Masson staining and fibrosis-related proteins E-cadherin, Collagen I, α-SMA, SMMHC -II immunohistochemical staining can evaluate the degree of pelvic fibrosis in mice with endometriosis.