动物造模,过敏性结膜炎小鼠模型 z-bio 2022-09-08 346

　　Objective To establish a mouse model of allergic conjunctivitis under the conditions of different antigens, different stimulation routes and different ages of mice. The establishment provides new ideas and methods.

　　Methods Sixty-five SPF Balb/c mice were exposed to different antigens (ovalbumin, ragweed pollen, house dust mites), different stimulation routes (gavage, aerosol inhalation, subcutaneous injection), and different mouse ages (1 The mouse animal models of allergic conjunctivitis were established under the conditions of 2 weeks, 2 weeks, 3 weeks and 8 weeks. RT-qPCR was used to detect the relative expression levels of CCL5 mRNA and IL-17 mRNA in mouse conjunctiva, and flow cytometry was used to detect the percentage of IL-17 in CD4+ T cells in spleen single cell suspension.

　　Results Compared with the negative control group, the percentage of IL-17, CCL5 mRNA and IL-17 mRNA expression in the ovalbumin group, the house dust mite group and the ragweed pollen group all increased, and the ragweed pollen group had the most significant increase. Compared with the negative control group, the IL-17 percentage and IL-17 mRNA expression in the subcutaneous injection group, the gavage group, the aerosol inhalation group, and the positive control group all increased, and the expression of CCL5 mRNA in the gavage group was close to that of the negative control group. The expression level of the subcutaneous injection group was closer to that of the positive control group. The percentage of IL-17, CCL5 mRNA, and IL-17 mRNA expression levels in 1-week-old and 2-week-old mice were similar, but significantly lower than those in 3-week-old and 8-week-old mice.

　　Conclusion Ragweed pollen has better sensitization in the establishment of allergic conjunctivitis mouse model. Subcutaneous injection of antigen challenged in mice model of allergic conjunctivitis can achieve satisfactory results, although no obvious ocular symptoms and signs, but the systemic and local sensitization state. The immune response of 3-week-old Balb/c mice was close to that of adult Balb/c mice.