动物造模 z-bio 2022-09-09 291

　　Objective To quickly establish an animal model of KM mice with low immunity, and use a simple and rapid method to identify the establishment of the animal model.

　　Methods Cyclophosphamide was given orally at a dose of 100 mg/kg, hydrocortisone at a dose of 80 mg/kg, 60% ethanol solution at a dose of 20 mL/Kg, and UVA ultraviolet skin irradiation. (Long wave), the dose was 10J/cm2, each group was administered for 3 consecutive days, the body weight of the mice before and after the experiment was measured, the contents of IgG, IgA and IgM in the serum of the mice were measured, and the peripheral blood of the mice was measured by flow cytometry. The contents of B lymphocyte CD19 and T lymphocyte CD3 were analyzed by automatic hematology analyzer to analyze the peripheral blood cells of mice.

　　Results The hypoimmunity model of KM mice in the cyclophosphamide group and hydrocortisone group was established, but the hypoimmunity model in the 60% ethanol group and the ultraviolet irradiation group was not established. The contents of IgG, IgA, and IgM in KM mice and the analysis of peripheral blood B and T lymphocytes of mice, as well as the routine analysis of mouse blood, can be used as reference detection indicators for the establishment of the model of KM mice with low immunity.

　　Conclusion The immunocompromised animal model of KM mice established by continuous oral gavage of cyclophosphamide for 3 days and 100 mg/Kg is established. This index is simple and easy to obtain, the detection cost is low, the time period is short, and the detection efficiency is high.