动物造模,痔疮模型 z-bio 2022-09-13 340

　　Objective: To study the protective effect of hemorrhoid magnetization ointment on acetic acid-induced hemorrhoids in mice.

　　Methods: 40 SPF grade ICR mice were divided into normal group (10 mice) and model group (30 mice). The model group was established with 20% acetic acid to establish a hemorrhoid model. After 24 hours of successful modeling, the model group was divided into model group. , Hemorrhoids magnetization ointment group and Mayinglong musk hemorrhoids ointment group, and carry out corresponding treatment. After 11 days of treatment, perianal score was performed, the levels of IL-6, IL-1β and TNF-α in peripheral blood were detected by CBA kit, the content of nitric oxide (NO) in peripheral blood by Griess kit, and evaluated by hematoxylin-eosin (HE) staining Rectal histopathological changes.

　　Results: Compared with the normal group, the perianal score in the model group was significantly increased, the pathological damage of rectal tissue was serious, and the levels of IL-6, IL-1β, TNF-α and NO in peripheral blood were significantly increased; In the drug group, the perianal score was significantly decreased, the pathological damage of rectal tissue was significantly improved, and the levels of L-6, IL-1β, TNF-α and NO in peripheral blood were significantly inhibited.

　　Conclusion: Hemorrhoid magnetization paste has a good therapeutic effect on mouse hemorrhoid model, and this therapeutic effect is closely related to regulating the level of inflammation in the body.