动物造模,胸主动脉瘤/夹层模型 z-bio 2023-05-09 424

　　Objective: To explore a simple and rapid detection method to evaluate whether the mouse model of thoracic aortic aneurysm/dissection was successfully established.

　　Methods: Twenty-four 3-week-old male C57BL/6 mice were randomly divided into a model group and a control group. The mice in the model group were fed with normal diet and drinking water dissolved in β-aminopropionitrile (BAPN) for 4 weeks; the mice in the control group were given normal diet and drinking water. The body weight of mice was monitored dynamically, and the thoracic aorta and left common carotid artery of mice were detected by Vevo2100 small animal ultrasound at the 4th week of modeling, and the thoracic aorta of mice was observed by Micro-CT.

　　Results: Compared with the control group, the body weight of the mice in the model group decreased significantly at the 4th week of modeling; in the small animal ultrasound and Micro-CT detection model group, the thoracic aortic vessels of the model group mice were significantly enlarged into tumors; the small animal ultrasound model The detectable pulse wave velocity changed in the group, and the degree of arteriosclerosis increased significantly.

　　Conclusion: Small animal ultrasound can simply, quickly and safely evaluate the success of the establishment of the mouse thoracic aortic aneurysm/dissection model, dynamically observe changes in the size of thoracic aortic aneurysm/dissection, and dynamically observe the degree of arteriosclerosis in mice.