动物造模,痫前期大鼠模型 z-bio 2022-09-13 347

　　OBJECTIVE: To explore the method of establishing a rat model of preeclampsia.

　　Methods: Thirty-nine SPF grade pregnant Wistar rats were included, including 16 cases in the reduced uterine perfusion (RUPP) group, 10 cases in the RUPP+irrigation group, and 13 cases in the sham operation group. RUPP operation or sham operation were performed on the 14th day of pregnancy, respectively, and blood pressure, urine protein and the status of the offspring were monitored on the 18th day of pregnancy.

　　Results: (1) The effect of RUPP operation on pregnant mice: Compared with the sham operation group, the systolic blood pressure, diastolic blood pressure and mean arterial pressure of pregnant mice in the RUPP group and RUPP + irrigation group all increased to varying degrees, but the blood pressure increase in the RUPP group was unstable. , the diastolic blood pressure was not significantly different from the sham operation group; the blood pressure in the RUPP + irrigation group increased steadily, and was significantly higher than that in the RUPP group; the RUPP group was significantly higher than the sham operation group at 18d and 24h of pregnancy. (2) The effect of RUPP surgery on offspring: RUPP surgery significantly reduced the live birth rate and pup growth rate on the 20th day of pregnancy; RUPP surgery resulted in significantly lower fetal weight and placental wet weight than the sham-operated group, and the RUPP+watering group was the most obvious.

　　Conclusion: RUPP + irrigation method can induce stable PE clinical manifestations in rats, and it is recommended to be an improved RUPP modeling method.