动物造模,高尿酸血症模型 z-bio 2022-09-13 501

　　Objective: To establish a rat model of hyperuricemia, and to explore whether hyperuricemia can lead to secondary cardiovascular disease.

　　Methods: Thirty-two SPF male SD rats were randomly divided into normal control group (Group C), potassium oxonate model group (Group M1), potassium oxonate combined with high sugar and high fat diet model group (Group M2), oxygen The model group (Group M3) with potassium oxonate combined with yeast extract diet, 8 animals in each group, were continuously modeled for 3 weeks. By comparing the serum uric acid (UA), blood urea nitrogen (BUN), creatinine (Cr), insulin (INS), blood glucose (GLU), triglyceride of rats (triglyceride, TG) and other indicators, supplemented by pathological examination results to study the rat model of hyperuricemia.

　　RESULTS: The hyperuricemia model rats replicated by intragastric administration of 750 mg/kg body weight potassium oxonate combined with yeast extract diet had significantly increased UA levels (P<0.01), long maintenance time, and 3/8 of the rats. The characteristics of renal lesions, hyperuricemia was accompanied by changes in GLU, INS and TG levels and heart lesions in 3/8 rats, suggesting the occurrence of secondary cardiovascular lesions.

　　Conclusion: The modeling method of potassium oxonate gavage combined with yeast extract feed is more suitable for the establishment of long-term hyperuricemia model in rats than simple potassium oxonate modeling, and it is also accompanied by the occurrence of secondary blood sugar disorders. The modelling scheme can be used in animal models for the study of the interaction mechanism between hyperuricemia and cardiovascular disease, and further applied to the preclinical pharmacodynamics comprehensive evaluation of the therapeutic drugs for hyperuricemia.