动物造模 z-bio 2022-09-13 320

　　OBJECTIVE: To provide experimental methods and technical support for the establishment of human coxsackie B2 virus infection model in rhesus macaques by preliminarily analyzing the infection characteristics of human coxsackie B2 virus in rhesus monkeys through oral infection, and for the follow-up development of its infection model. Lay the foundation for the research on infection mechanism and pathophysiology.

　　Methods: Coxsackie B2 virus was used to infect rhesus monkeys aged 3 to 4 months by oral infection, and the clinical symptoms and body temperature of rhesus monkeys were observed and recorded; blood was collected to detect physiological and biochemical indicators; blood samples and herpes were detected for virus; herpes tissue Perform pathological testing.

　　Results: The rhesus monkeys developed herpes on the hands, feet and mouth 2-5 days after infection; the animals showed depression, decreased activity and food intake; body temperature showed a heat retention curve; Coxsackie was detected in the herpes fluid and blood samples B2 virus; blood routine test showed leukopenia, increased monocytes, decreased lymphocytes, increased granulocytes, increased eosinophils, and decreased basophils. Blood biochemical tests showed that liver function, renal function, and myocardial enzymes all increased to varying degrees; herpes histopathology showed that rhesus monkey herpes showed thickening of squamous epithelium, surface hyperkeratosis, local necrosis with inflammatory cell infiltration, abscesses form.

　　Conclusion: Human coxsackie B2 virus infected infant monkeys with a series of characteristic hand, foot and mouth herpes, clinical symptoms, physiological and biochemical conditions, viremia, etc., indicating that oral infection in rhesus monkey infants can successfully construct coxsackie The animal model of B2 virus infection, and the relevant detection methods and technologies established are feasible, which lays the experimental foundation for the subsequent establishment and development of the rhesus coxsackie B2 virus infection model, pathogenesis research, and evaluation of drugs and vaccines.