动物造模,夹层动脉瘤动物模型 z-bio 2022-09-13 422

　　Objective: To explore the effect of β-aminopropionitrile on the wall of the aorta in mice, and to compare the pathogenesis of human dissecting aneurysm, in order to further explore the animal model of dissecting aneurysm with the characteristics of human disease.

　　Methods: BAPN was mixed into the drinking water of experimental animals to prepare BAPN solutions with concentrations of 0.2%, 0.4% and 0.6%. After the experiment was over or the animals died, the animals were dissected, their aortas were separated, and the general changes were observed. The aorta was divided into four parts: ascending aorta, descending aorta, upper abdominal aortic renal artery and lower abdominal aortic renal artery. HE staining was performed on the cross-section of the segmental blood vessels, and various indicators such as the inner diameter of the blood vessel, the area of the media, etc. were measured. At the same time, the large arteries of the patients with type A dissecting aneurysm who underwent thoracotomy were selected for HE staining to observe the pathological changes and their correlation with the occurrence of dissection. Aneurysm in rat aorta for comparison.

　　Results: 1) BAPN could significantly affect water intake and body weight gain in rats or mice. 2) BAPN could induce dilation of large arteries, thickening of media, decrease elastin, and disorder in rats or mice, and its pathological changes were consistent with human dissection Characteristics of pathological changes of aneurysms. 3) BAPN solution with a concentration of 0.4% has the highest success rate.

　　CONCLUSION: The C57BL/6 mouse model of dissecting aneurysm can be used as a simple, economical and effective animal model for further research; SD rats have higher rates of intestinal rupture, scoliosis and other systemic pathological changes than dissecting aneurysms However, it needs further exploration as an animal model of dissecting aneurysm.