动物造模,炎症模型 z-bio 2022-09-13 405

　　Objective: To analyze the pathogenicity of influenza A virus using a mouse model of inflammation.

　　Methods: Three different influenza A viruses A/swine/Jiangsu/C1/08(H9N2)(H9C1)､A/swine/Shandong/731/2009(SD731)､A/Puerto Rico/8/34(H1N1) (PR8), 5-week-old BALB/c mice were intranasally infected with 1×106 TCID50, and the clinical symptoms were observed, and the viral load and inflammatory factor levels in the lung tissue were detected.

　　Results: In the pathogenicity test of BALB/c mice, the level of inflammation in lung tissue and virus replication caused by SD731 and PR8 viruses were significantly higher than those of H9C1. The lethality rate of SD731 was 80% and that of PR8 was 100%. weight loss, but the mice did not die.

　　Conclusion: The inflammatory model of influenza virus-infected mice was successfully established.