动物造模,抑郁模型 z-bio 2022-09-13 382

　　Objective: To establish a depression model in C57BL/6 mice, and to preliminarily explore the effect of high-frequency sound waves in Mozart's K448 sonata on improving depressive symptoms in C57BL/6 mice.

　　Methods: 1) Establishment of chronic stress model: Mice with large differences in the number of activities were excluded based on the results of autonomous activity experiments, and the rest were divided into blank group (n=10), model group (n=36), model group after 5 weeks Chronic unpredictable and mild stress (CUMS) was used to establish a mouse model of depression. (2) Therapeutic intervention: After the model was successfully established, the mice in the model group were randomly and balancedly divided into the model control group (n=12), fluoride The fluoxetine group (n=12) and the music group (n=12). The fluoxetine group was given intraperitoneal injection of fluoxetine hydrochloride solution (10 mg/kg) every day, and the other two groups were given the same amount of normal saline. The music group was given 2 hours a day. High-frequency music intervention, the other two groups did not receive music intervention. The intervention lasted for 2 weeks. (3) Effect evaluation: 3 days before the experiment and every week during the experiment, the body weight was weighed and recorded, the first week, the fifth week, and the seventh week of the experiment. The tail suspension test (TST) and the forced swimming test (FST) were performed. After the behavioral experiment at the 7th week, the mouse brain tissue was taken to prepare a homogenate, which was subjected to enzyme-linked immunosorbent assay (ELISA) method. The content of brain-derived neurotrophic factor (BDNF) was determined by ELISA.

　　Results: (1) The CUMS mouse model was successfully established. At the 5th week, the immobility time of the tail suspension of the mice in the model group increased significantly, and the difference was significant (P<0.01). The immobility time of forced swimming increased, and the difference was significant (P <0.05). (2) Compared with the model control group, the immobility time of the fluoxetine group was significantly shortened in the tail suspension test, and the difference was significant (P<0.01). p="">0.05); compared with the model control group, the tail suspension immobility time was shortened in the music group, and the difference was significant (P<0.05). . Compared with the blank group, the BDNF content in the brain tissue homogenate of the model control group was significantly decreased, and the difference was significant (P<0.01). The content of BDNF increased significantly, and the difference was significant (P<0.01), but="" there="" was="" no="" significant="" difference="" between="" the="" music="" group="" and="" model="" control="" p="">0.05).

　　Conclusion: Mozart's K448 sonata high-frequency sound waves can optimize the therapeutic effect of the mouse model of depression to a certain extent.