动物造模,肺成纤维细胞模型 z-bio 2022-09-13 309

　　Objective: To explore the feasibility of establishing an experimental model of CA16-infected tree shrew lung fibroblasts.

　　Methods: Enterovirus CA16 was used to infect TSLF, and human lung fibroblasts (KMB-17) were used as control. The viral load was detected by real-time fluorescent quantitative PCR with needle method, the relative expression of the receptor SCARB2 gene was detected by real-time fluorescent quantitative PCR with β-Actin as an internal reference dye, and its correlation with infection was analyzed combined with gene sequence comparison.

　　RESULTS: CA16 infection of TSLF could cause obvious cytopathic changes. Immunofluorescence experiments showed that the virus and receptor SCARB2 protein were infected with TSLF at a ratio of 100TCID50/105cells, and the viral load reached the highest at about 48h. expression, and its gene sequence also has high homology with human.

　　Conclusion: CA16 can infect TSLF, and tree shrew SCARB2 can be involved in the infection.