动物造模,哮喘模型 z-bio 2022-09-13 407

　　Objective: The mechanism of traditional Chinese medicine to treat diseases by regulating the flora has been gradually discovered, and to explore the establishment of an asthma model of respiratory flora disorder caused by antibiotics, in order to provide a suitable model for the study of the effect of traditional Chinese medicine on respiratory flora and asthma.

　　Methods: Forty SPF male BALB/c mice were randomly divided into the following 4 groups: normal control group, asthma model group, asthma model group with intranasal antibiotics, and asthma model group with intraperitoneal injection of antibiotics. House dust mite (house dust mite) , HDM) to establish an asthma model, and the antibiotic cefoperazone sulbactam sodium (CFP) was administered by intranasal or intraperitoneal injection, respectively, to observe the effect of antibiotics on HDM-induced asthma in mice. The asthma indexes of mice in each group were counted: eosinophils (EOS) in blood were counted, IgE levels in serum were detected, bronchoalveolar lavage fluid (BALF) in mice and lung tissue homogenate were detected. The levels of TH2-type cytokines IL-5 and IL-13 were detected by HE, Masson and PAS staining in the lung tissue of mice to observe the histopathological changes.

　　Results: The levels of EOS in blood, IgE in serum, BALF and IL-5 and IL-13 in lung tissue homogenate of mice in asthma model group were significantly higher than those in blank group (P<0.05). The model group had a more significant increase (P<0.05), and histopathological observation also found that the inflammatory infiltration, extracellular matrix deposition and mucus secretion were more serious in the intranasal antibiotic model group, while the intraperitoneal antibiotic group was associated with asthma. There was no significant change in the indicators compared with the model group.

　　Conclusion: The intranasal administration of the antibiotic cefoperazone and sulbactam sodium to mice can aggravate HDM-induced asthma in mice, suggesting that the administration of antibiotics to the respiratory tract may lead to the disturbance of respiratory flora and aggravate the occurrence of asthma. This model can be used as a model without feeding GF. A tool for studying traditional Chinese medicine in regulating respiratory microbiota and asthma in mice.