动物造模,结肠癌肝转移裸鼠模型 z-bio 2022-09-14 302

　　OBJECTIVE: To construct a colon cancer liver metastasis model with high metastasis rate, simple operation and reliable results for experimental research on the prevention and treatment of colon cancer metastasis.

　　Methods: Fifteen Balb/c nude mice were divided into 3 groups (group A, group B, group C), and 5 Balb/c mice were divided into group D alone. The colon cancer liver metastasis model was established by the spleen implantation and spleen preservation method and the spleen incision method in 0.2 mL of the suspension, respectively.

　　RESULTS: The successful rate of modeling nude mice in group A was 100% (5/5). Both liver and spleen formed tumors. The number of liver metastases was less and more scattered, and most of them were distributed in the right lobe of liver. The average survival time was (26.6±3.4 )d; the success rate of modeling nude mice in group B was 40% (2/5), the metastases were scattered on the liver surface, the volume was larger than that in group A, and the average survival time was (36.8±4.2) d; the modeling of nude mice in group C was successful The rate was 100% (5/5), both the liver and spleen formed tumors, the number of liver metastases was large, and multiple metastases fused into clusters, occupying the entire right lobe of the liver, and the average survival time was (20.2±2.6) d; group D No metastases were found in the liver. Some nude mice in the three groups had intraperitoneal metastases (2 in group A and 3 in group C), and no heart, lung, brain, and kidney metastases were found. Histological and cytological morphology of liver metastases in nude mice in three groups Consistent with adenocarcinoma features.

　　Conclusion: The spleen-preserving method can obtain a high success rate of modeling, and can effectively simulate the pathway and process of human colon cancer cells metastasize to the liver through blood.