动物造模,鹌鹑痛风模型 z-bio 2022-09-14 301

　　Objective: By simulating the clinical high-protein and high-calcium diet to induce gout in Difak quail, the method of establishing the gout model of quail was preliminarily discussed, and the basis for the research on the pathogenesis of gout and the screening of prevention and treatment drugs were provided.

　　Methods: Thirty-six 30-day-old male healthy Difak quails were selected and randomly divided into 3 groups according to body weight and serum uric acid level, namely control group, model group I and model group II, with 12 in each group. Feeding basis of quail in control group The model group was fed a high-protein and high-calcium diet (the crude protein content of the diet was 29.5%, and the calcium content was 7.8%). The serum and fecal uric acid levels of quails were detected, and renal function was detected at the same time, and histological and pathological sections were observed.

　　Results: High-protein and high-calcium diets could significantly increase the level of serum uric acid in quails; significantly reduce the level of fecal uric acid; increase the levels of serum creatinine and blood urea nitrogen; lead to obvious urate deposition in the kidneys of quails. The combination of high-protein and high-calcium diets Restricted drinking water can aggravate the increase of serum uric acid level and the deposition of urate in the kidneys, and cause some quails to have gout symptoms such as joint swelling and deformation.

　　Conclusion: The gout quail model can be established by feeding high-protein and high-calcium diets and limiting water intake, and its pathogenesis is more in line with the clinical etiology, pathogenesis and clinical manifestations of gout, and provides a reference for the basic and drug development research of gout.