动物造模,内脏高敏感模型 z-bio 2022-09-14 335

　　OBJECTIVE: To establish a rat model of visceral hypersensitivity by plantar electric shock, and to evaluate its effectiveness and advantages.

　　Methods: Female SD rats were randomly divided into normal control (NC) group, foot shock (FSS) group and water avoidance stress (WAS) group. The NC group was placed in a simulated stress box for 1 h every day without stimulation; FSS The group was placed in the electric shock box for 1 h every day, and given electric shock stimulation, the stimulation voltage was 40V, the stimulation frequency was 20 times/min, 1 s each time, for a total of 5 min; the WAS group was given 1 h of water avoidance stress every day; the modeling period was 10 d. Observation. The general condition and defecation of the rats were recorded, the abdominal wall retraction reflex (AWR) was used to detect the visceral sensitivity, and the serum CRF, ACTH, CORT, 5-HT and colon tissue 5-HT concentrations were detected by ELISA.

　　Results: (1) The visceral sensitivity of the FSS group was significantly higher than that of the WAS group and the NC group (P<0.05), and there was no obvious pathological damage to the colonic mucosa. (2) The serum CRF, ACTH, CORT, 5 of the FSS group was The concentration of -HT and 5-HT in colon tissue were significantly higher than those in the WAS group and the NC group (P<0.05). (3) The defecation volume of the rats in the FSS group was significantly higher than that in the WAS group and the NC group (P<0.05). The fecal water content was significantly higher than that in the NC group (P<0.05), and the defecation volume and fecal water content of the rats were stable.

　　CONCLUSION: The foot shock modeling method can establish a rat model of visceral hypersensitivity and is superior to the water avoidance stress method.