动物造模,大鼠恐惧应激模型 z-bio 2023-06-07 780

　　Objective: To construct a rat model of different degrees of fear stress, and to explore the effect of fear stress on the visual cognitive ability of LE rats.

　　Methods: Using plantar electric shock as a stress stimulus, a cognitive choice experiment was designed, and the neural response signals in the amygdala brain area were collected for functional network analysis to evaluate the visual cognitive effect of the rats. First, the experimental rats were divided into strong (S+) and The weak (S) fear stress group and the control group (N) were set with different intensities of plantar electric shock stimulation respectively; then they were subjected to visual cognitive strengthening training with a single figure "△"; ” and “Ten” figures) to conduct visual choice test experiments. In addition, combined with the complex network theory, the visual cognitive function network of the amygdala nuclei of fear stress rats was constructed, and the brain function network was characterized by the average path length and clustering coefficient. information transfer efficiency.

　　Results: The time required to complete the visual cognitive intensive training was significantly higher in the S+ group than in the S and N groups. In the early stage of intensive training, the S group was significantly higher than the N group, and there was no significant difference between the two groups in the later stage. In the brain function network analysis, effective visual information transmission was formed between the amygdala neurons in the S group and the N group, but not in the S+ group.

　　Conclusion: Fear stress has a negative impact on visual cognition, and the cognitive effect becomes significantly worse with the increase of fear level.