动物造模 z-bio 2023-06-14 669

　　Objective: To establish a model of oral mucositis in golden hamsters induced by 5-fluorouracil combined with mechanical scratching.

　　Methods: Male golden hamsters were divided into control group and model group. Animals in model group were injected intraperitoneally with 60 mg/kg and 40 mg/kg of 5-fluorouracil (5FU) on day 1 and day 2, respectively, and on day 4. The right cheek pouch of the golden hamster was everted, and an 18-gauge needle was used to form a superficial scratch in the oral cavity. The animals in the control group were injected with an equal volume of normal saline on the 1st and 2nd day respectively. Modeling The macroscopic changes of the right cheek pouch of the animals in each group were observed and scored every day, and the body weight and the average daily food intake of each animal were recorded at the same time. Blood was collected on the 8th and 14th days after modeling to determine the pro-inflammatory cytokine TNF in serum. -α and IL-1β contents, the right cheek pouch tissue was taken for HE staining to determine the malondialdehyde (MDA) content and superoxide dismutase (SOD) activity.

　　Results: Compared with the control group, the golden hamsters in the model group had ulcers on the right cheek pouch tissue on the 3rd day after modeling and had the highest macroscopic score. The average daily food intake and body weight of the animals decreased significantly within 8 days after modeling. On the 8th and 14th days after modelling, the tissue structure of the right cheek pouch of golden hamster was damaged, showing an inflammatory reaction, the contents of TNF-α and IL-1β in serum were significantly increased, and the content of MDA in the cheek pouch tissue was significantly increased. high, the SOD activity was significantly reduced.

　　Conclusion: 5-Fluorouracil combined with mechanical scratching can lead to the formation of oral mucositis in golden hamsters. The inflammatory reaction is obvious within 8 days after modeling, and the most obvious on the third day. It can be used for the study of the pathogenesis and therapeutic drugs of oral mucositis.