动物造模 z-bio 2022-09-14 349

　　Objective: To study the physical fatigue in mice caused by sleep disturbance at different times, and to provide a stable and reliable animal model for the research and development of products for relieving physical fatigue.

　　Methods: After different time (5, 10, 15d) sleep disturbances were performed on the mice using the roller sleep disturbance instrument, the animals in each group were subjected to the wheel running experiment and the weight-bearing swimming experiment, and the contents of liver glycogen and serum urea nitrogen in the mice were determined. .

　　Results: In the wheel running experiment, the sleep disturbance 5d, 10d, and 15d groups showed a decreasing trend compared with the control group, but the difference was not significant (P>0.05). The time to exhaustion was significantly shortened (P<0.05, P<0.01), and the number of sinking and the total sinking time were significantly reduced (P <0.0 P <0.01). Compared with the control group, the 5d, 10d, and 15d interference groups had higher levels of hepatic glycogen. Compared with the control group, the serum urea nitrogen content in the interference group for 10 days and 15 days was significantly increased (P<0.05).

　　Conclusion: Sleep disturbance by roller method (rolling parameter 3r/min, 1min per revolution, 1min interval) for 10 and 15 days can induce the behavioral performance of physical fatigue in mice.