动物造模,果蝇心衰模型 z-bio 2022-09-14 382

　　Objective: To establish a model of Drosophila heart failure induced by high-fat diet.

　　Methods: The wild-type Drosophila w1118 was selected as the experimental object, and the Drosophila were fed with different concentrations of coconut oil (0%, 7.5%, 15%, 30%) for 5 days, and the triacylglycerol content in the Drosophila was detected. Follow-up In the experiment, the non-high-fat feeding group (0% coconut oil) was used as the control, and the 30% coconut oil high-fat feeding group was used as the experimental group. The negative geotaxis test was used to detect the exercise ability of Drosophila. Drosophila adult heart function analysis platform, photographing fruit flies The heart beats of flies under semi-anatomical conditions (30s) were analyzed by software to obtain adult cardiac function indicators, including heartbeat cycle, systolic and diastolic diameter, cardiac contractility index, etc. Real-time quantitative PCR was used to detect RNA expression in the hearts of high-fat-fed Drosophila Variety.

　　Results: Compared with non-high-fat-fed Drosophila, the content of triacylglycerol in different proportions of high-fat-fed Drosophila increased with the concentration of coconut oil (P < 0.05); high-fat diet significantly affected Drosophila activity (P < 0.001). ); the heartbeat cycle of high-fat-fed Drosophila was shortened (P< 0.01), and the cardiac contractility was weakened (P< 0.001); real-time quantitative PCR found that some genes involved in lipid metabolism in high-fat-fed Drosophila fa2h, CG6277, CG3699 , CG9914, the expression of lip2 was significantly down-regulated (P < 0.05).

　　Conclusion: High-fat diet seriously affects Drosophila exercise ability and adult heart function, and a high-fat diet-induced Drosophila heart failure model was successfully established.