动物造模,性早熟痰湿壅滞证大鼠模型 z-bio 2022-09-14 270

　　OBJECTIVE: To explore the method of systematic review of the rat model of precocious puberty with phlegm-damp stagnation syndrome.

　　Methods: By observing the syndrome manifestations of female SD rats in the precocious puberty phlegm-damp stagnation group, the precocious puberty non-phlegm-damp stagnation group, the blank control group, the parental model group, and the parental control group, combined with the data of the open field experiment, to explore the disease A systematic review of evidence-based animal models.

　　Results: Compared with the parental control group, the female mice in the parental model group had no significant difference in body weight gain (P> 0.05). Among the 4-week-old female mice, the female mice in the precocious puberty phlegm-damp stagnation group lost significantly more weight than the blank group (P<0.05). Compared with the precocious puberty non-phlegm-dampness stagnation group, the body weight was reduced (P<0.05). The total movement distance and movement speed of female SD rats in the parental model group were significantly lower than those in the parental control group (P<0.05). The resting time was significantly higher than that of the parental control group (P<0.05), while="" there="" was="" no="" significant="" difference="" in="" the="" movement="" resting="" times="" and="" time="" among="" three="" groups="" of="" offspring="" p="">0.05).

　　Conclusion: High-fat diet did not increase the body weight of female SD rats, and the body weight index may not be the key index for the systematic evaluation of the animal model of phlegm-damp stagnation syndrome; the behavioral changes of the rat model of phlegm-damp stagnation syndrome are similar to the clinical manifestations of this syndrome The open-field experiment can be used to further study the evaluation method of animal models of phlegm-damp stagnation syndrome. No behavioral differences were found between the groups of female offspring.