动物造模,薄型子宫内膜小鼠模型 z-bio 2022-09-14 284

　　Objective: To further explore the feasibility of establishing a stable thin endometrium mouse model by intrauterine injection of 95% ethanol by means of immunohistochemistry and fertility assessment, and to provide information for the related research on the pathological characteristics and repair mechanism of thin endometrium. Ideal animal model.

　　Methods: Seventy-eight female C57BL/6J mice that were sexually mature and had regular estrus were selected as experimental animals, and a thin endometrial mouse model was established by injecting 95% ethanol into the uterine cavity to damage the endometrium. The 78 experimental mice were randomly divided into two groups. The mice were divided into two groups, one group of 30 mice, and they were randomly divided into blank group, control group and experimental group. In the third estrus period after modeling, the experimental mice were sacrificed by cervical dislocation, and uterine samples were collected for immunohistochemical detection. The expression of cytokeratin, vimentin, vascular endothelial growth factor and estrogen receptor α was used to evaluate the regeneration of endometrial cells; another group of 48 rats were randomly divided into blank group, control group, unilateral injury group and In the bilateral injury group, all surviving mice were co-mated in the third estrus period after modeling to analyze the effect of thin endometrium on mouse fertility, and to evaluate whether the thin endometrium mouse model was successfully established.

　　Results: Immunohistochemical results showed that the expressions of endometrial cytokeratin, vimentin, vascular endothelial growth factor and estrogen receptor α in the experimental group injected with 95% ethanol were significantly lower than those in the blank group and the control group . Fertility evaluation experiment showed that after co-co-mating, the mice in the blank group and the control group conceived normally, the mice in the bilateral injury experimental group did not conceive, the injured uterus in the unilateral injury experimental group did not conceive, and the uninjured uterus conceived normally, and The average pregnancy rate of the injured uterus was significantly lower than that of the uninjured side (P < 0.05), indicating that intrauterine injection of 95% ethanol could damage the endometrium of mice, resulting in decreased fertility.

　　Conclusion: This study successfully established a thin endometrial mouse model, which can be used to study the repair and repair mechanism of thin endometrial.