动物造模,肢体缺血-再灌注损伤模型 z-bio 2022-09-14 223

　　Objective: To introduce an improved method of blocking femoral artery blood flow in vitro, and to establish a simple, controllable and reliable model of limb ischemia-reperfusion injury in rats.

　　Methods: Fifteen 2-month-old SPF male SD rats were randomly divided into 5 groups: normal group, ischemia-reperfusion 0h group, 2h group, 4h group and 8h group. The normal group did not block blood flow, and the other 4 groups The ischemia-reperfusion injury model was constructed, the morphological changes of skeletal muscle cells were observed by HE staining, and the apoptosis was detected by DAPI method.

　　RESULTS: Except for the normal group, all groups had different degrees of muscle fiber swelling, irregular arrangement, and widening of muscle fiber gaps. The injury degree of the 4h and 8h groups was significantly worse than that of the 0h and 2h groups, and the 8h group had the most serious injury. , and the four groups of skeletal muscle cells showed typical morphological characteristics of apoptosis.

　　Conclusion: This method can reflect the pathological changes of skeletal muscle during LIRI, and is similar to the clinical characteristics of LIRI. It is simple and efficient, and can be used for the construction of animal models of limb ischemia-reperfusion.