动物造模,肺腺瘤小鼠模型 z-bio 2022-09-14 253

　　Objective: To establish a mouse model of lung adenoma and conduct blood biochemistry, blood routine, urine routine and pathological detection.

　　Methods: 7 B6.129S4-Krastm4Tyj/J positive mice were identified by PCR, 4 experimental groups, 3 control groups, and 3 wild-type control groups. The experimental group and the wild-type control group were instilled with Cre adenovirus. Once a week, a total of 4 times. In the 5th week, blood and urine were collected for blood biochemistry and blood routine examination, and lung tissue was collected for pathological detection and fluorescence quantitative PCR to detect Cre expression.

　　RESULTS: All 4 positive mice developed multiple lung adenomas, with an average number of 4.25 and a volume of (10.81±6.38) mm3. No tumor was found in the control group. The tumor grew expansively, with clear borders, and the tumor cells were cubic epithelioid. The arrangement was irregular glandular tube, and no mitoses were seen. Compared with the control group, the low-density lipoprotein cholesterol and mean platelet volume of the lung adenoma mice increased significantly, and the difference was significant; the direct bilirubin increased compared with the control group. Compared with the control group, high and high-density lipoprotein cholesterol decreased, and the difference was significant.

　　Conclusion: A mouse model of lung tumor was established, and its blood biochemistry, blood routine, urine routine and pathology were measured.