动物造模,谵妄模型 z-bio 2022-09-14 270

　　OBJECTIVE: To establish a mouse model of delirium by detecting scopolamine with a combined behavioral method.

　　Methods: To compare the behavioral changes of the two groups of mice: the delirium group (n=12) and the control group (n=8) were injected with 15 mg/kg scopolamine and an equal volume of normal saline by intraperitoneal injection, respectively, within 30-60 min after the injection. The two groups of mice were subjected to the black-and-white box experiment for 10 minutes, the open field experiment for 10 minutes, and the non-selective non-sustained attention experiment for 10 minutes—combined behavioral experiments. The five behavioral indicators of the two groups of mice were compared to detect delirium.

　　Results: Compared with the control group, the delirium group mice had significant differences in 5 behavioral indexes, the mice stayed in the white box for a significantly shorter time, P<0.05; the mice's activity speed was significantly increased, P<0.05 0.0 significantly increased the activity time along the wall, P<0.0, the freezing time was significantly decreased, P<0.05; the mouse "attention" level decreased significantly, P<0.05.

　　Conclusion: The combined behavioral experiment method can reflect the diagnostic elements of delirium, better reflect the delirium state, and it takes a shorter time.