动物造模,幼羊颅骨缺损模型 z-bio 2022-09-15 273

　　OBJECTIVE: To detect the expression of Dlx2 gene in the skull tissue of the small-tailed Han sheep model of skull defect, and to explore its significance to the skull defect.

　　METHODS: Ten 1-month-old Small Tail Han sheep were selected to establish a skull defect model, and the neonatal skull tissue at the defect site and the native skull tissue adjacent to the defect site were collected. Real-time PCR and Western blot techniques were used to detect the mRNA and protein expression levels of Dlx2. .

　　RESULTS: Compared with the native skull tissue adjacent to the defect site, the Dlx2 mRNA and protein expression levels in the neonatal skull tissue at the defect site were significantly increased (P<0.05).

　　Conclusion: The increased expression of Dlx2 gene may have important significance for the repair of skull defects.