动物造模 z-bio 2022-09-15 279

　　OBJECTIVE: To construct two models of renal calculi in rats, and to provide support for the exploration of the etiology of nephrolithiasis.

　　Methods: Sixty rats were randomly divided into Nanobacteria (NB) group (NB group, 1.2mL NB suspension was injected into tail vein, 30 rats) and ethylene glycol group (EG group, 1.25% ethylene glycol drinking water + 1 % ammonium chloride 2 mL/d by gavage, the duration of gavage was 2w, 30 rats), the experimental period was 10w, 3 rats in each group were sacrificed every week, and blood, urine and kidney samples were collected from the rats before sacrifice.

　　Results: The general conditions of the two groups of rats were significantly different, but the ratio of kidney to body was not significant; the difference in the number of pathological crystals was not significant; The difference was not significant (P>0.05); the difference in urinary calcium, urine pH, urine specific gravity and 24h urine volume was not significant (P>0.05).

　　Conclusion: There is no significant difference between the two modeling methods in terms of research indicators, indicating the feasibility of nanobacteria modeling. Compared with the traditional ethylene glycol modeling method, NB modeling has the advantage of being more gentle, And NB is derived from the kidney stone itself, this modeling method is more similar to the formation of human kidney stone, and is more conducive to the study of the cause of kidney stone.