动物造模,化疗性肠黏膜炎模型 z-bio 2022-09-16 309

　　Objective: To study the 5-fluorouracil-induced mouse model of chemotherapy-induced intestinal mucositis.

　　METHODS: Different doses of 5-FU were injected into mice by intraperitoneal injection for a single time or for 5 consecutive days. The body weight and diarrhea of mice were observed every day, and the peripheral blood of mice was observed at 72 h or 24 h after the last administration, respectively. Histopathological changes of the small intestine.

　　Results: Compared with the normal group, after a single or continuous 5-day administration of 5-FU, the mice in each dose group had different degrees of diarrhea and weight loss, and the levels of leukocytes and platelets in peripheral blood were significantly reduced (P ＜ 0.05 or P ＜ 0.05 or P ＜ 0.05). 0.01), in which the single-dose 400 mg/kg group and the continuous 50, 100 mg/kg group had obvious pathological characteristics of intestinal mucositis, and the 100 mg/kg group was too high, and the mortality rate was 100%.

　　CONCLUSION: The effect of 5-FU in a single or continuous 5-day-inducing mouse intestinal mucositis is dose-related, in which 400 mg/kg is the appropriate dose for single administration, and 50 mg/kg for 5-day continuous administration. appropriate dose.