动物造模,胰腺癌原位移植模型 z-bio 2022-09-16 420

　　Objective: To establish an orthotopic transplantation model of pancreatic cancer based on clinical tumor specimens, and to carry out model evaluation research.

　　Methods: The clinical fresh surgical specimens of pancreatic cancer were transplanted into the pancreas of nude mice to establish an orthotopic xenograft model (PDOX), and the tumor growth status was evaluated by in vivo imaging technology and PET/CT; STR technology was used to analyze the source of the transplanted tumor. The pathological characteristics of xenograft tumors were determined by histomorphological observation and immunohistochemistry, and the expression level of CA19-9 in peripheral blood of PDOX model was also detected.

　　RESULTS: Six weeks after the model was established, the near-infrared fluorescence signal was observed to be significantly enriched in the tumor site by in vivo imaging, and the location and size of the tumor could be preliminarily determined by the fluorescence intensity; the abdominal 18F-FDG could be clearly observed by small animal PET/CT. The molecular probe-enriched area was consistent with the expected tumor location and size; after euthanasia, the isolated organ tissues and tumor tissues were dissected, and the tumor growth status was further confirmed by near-infrared fluorescence imaging; STR detection confirmed that the transplanted tumor was of human origin The sex ratio was 99.99%; histomorphological and immunohistochemical analysis showed that the transplanted tumor grew in the pancreas of nude mice; the detection of CA19-9 in serum further confirmed the occurrence of pancreatic cancer.

　　Conclusion: The human pancreatic cancer orthotopic xenograft model was successfully established, and the model was comprehensively evaluated by small animal in vivo imaging, PET/CT and other techniques, which provided a good animal model for the pathogenesis and treatment of pancreatic cancer.