动物造模,肾缺血再灌注损伤模型 z-bio 2022-09-16 223

　　Objective: To explore a method for making a rat model of renal ischemia-reperfusion injury with less injury, simple operation and stable effect.

　　Methods: SD male rats were randomly divided into normal group (C group), sham operation group (S group) and experimental group (IR group). The rats in the experimental group entered the bilateral retroperitoneal space through the back muscle fascia through the median skin incision on the back, separated the bilateral renal pedicles, clamped the bilateral renal pedicles with non-injury miniature arterial clips for 50 min, then released them, and recovered. perfusion. Rats in the sham operation group did not clip the renal pedicle, and the rest of the steps were the same as those in the experimental group; the rats in the normal group were only anesthetized. The survival status, serum creatinine, blood urea nitrogen and renal tissue structure changes were observed 24 hours after operation.

　　Results: In the IR group, the skin incision was (2.24 ± 0.27) cm, the right lumbar back fascia and muscle incision was (1.36 ± 0.21) cm, and the left low back fascia and muscle incision (1.36 ± 0.24) cm. It took (3.30 ± 0.37) min from skin incision to clipping of both renal pedicles. IR group modeling success rate of 95%. Compared with the normal group and the sham operation group, the levels of serum creatinine and blood urea nitrogen in the IR group were significantly increased (P ＜ 0.01), and the renal tubular injury score was significantly increased (P ＜ 0.05).

　　Conclusion: The model of renal ischemia-reperfusion injury was established by the dorsal-entry retroperitoneal double-renal pedicle clipping method, with stable effect, high success rate, small incision, less bleeding, light stimulation to animals, and easy operation.