动物造模,脓毒症模型 z-bio 2022-09-16 349

　　Objective: To investigate the changes of coagulation function in a rat model of sepsis induced by cecal ligation and puncture.

　　METHODS: CLP was used to induce sepsis in SD rats, and relevant coagulation function indexes were detected at 8, 16 and 48 h after operation, and the histopathological changes of lung, kidney, liver and spleen were observed by HE staining.

　　Results: The 12-day survival rate of CLP-induced sepsis rats was 30%, with acute onset and high mortality. In the acute stage of disease development, CLP rats showed prolonged APTT time at 8 hours (P<0.05), and at 16 hours The time of PT was prolonged (P<0.05), and the activity of XII of the intrinsic coagulation pathway and the activity of VII of the extrinsic coagulation pathway were temporarily inhibited. TT was prolonged at 48h (P<0.01). The content of FIB gradually started from 16h. increased (P < 0.001). Among other important indexes related to coagulation and anticoagulation, the amount of PLT decreased gradually from 8 hours (P < 0.01); the amount of vWF:Ag increased gradually from 8 hours (P < 0.001); the amount of D-D increased from 8 hours 16h gradually increased (P<0.05); PS:Ag content decreased significantly until 48h (P<0.001); but AT-III content did not change significantly. Pathological examination showed that lung, kidney, liver, and spleen tissue were damaged to varying degrees. , but did not show obvious features of venous thrombosis and bleeding.

　　Conclusion: The rat sepsis model has coagulation disorder in the acute phase, but no histopathological changes caused by coagulation function are observed.